Fertilized pre-implantation embryos frozen in liquid nitrogen can be thawed and implanted into pseudopregnant recipient females in order to rederive a transgenic mouse line. Alternatively, pre-implantation embryos can be harvested from time-mated females, “washed”, and re-implanted into pseudopregnant recipient females. This technique eliminates viral, bacterial, and parasitic infections, and is commonly used to convert mouse colonies to Specific Pathogen Free (SPF) status. Embryo rederivation is also the preferred method of importing mouse lines from non-approved vendors to the UW.
- generate pseudopregnant recipient females
- thaw embryos and surgically transfer to pseudopregnant recipient females or
- collect embryos from super-ovulated females, wash embryos, and re-implant
- monitor pregnant females
- wean pups, ear-tag, and tail-tip for genotyping
- For any non-UW mice, the recipients must be tested for pathogens before the pups are transferred to your colony. The requesting lab is responsible for any testing fees.
- The cost of embryo thaw and rederivation includes thaw and transfer of one straw of embryos to time-mated pseudopregnant females (2-3 recipients).
- The cost of embryo harvest from infected females for SPF conversion includes embryo harvest from up-to 10 superovulated females and surgical re-implantation into pseudopregnant recipient females.
- When thawing embryos frozen in the TRP Core or fresh embryos collected from superovulated females, if the recipients do not become pregnant for any reason, we will repeat the procedure at no additional cost.
- The TRP will accept frozen embryos from outside institutions for rederivation, but please note that there is no guarantee of live pups being produced. We will do our utmost to produce live pups, but we have no way of controlling how the embryos were frozen, stored, handled, or shipped before we received them, therefore we cannot guarantee that the embryos will be viable for rederivation.
- Click here for current rates.