In Vitro Fertilization (IVF)
Mice can be rederived using in vitro fertilization (IVF). Sperm from a frozen sample or a donor male supplied by the investigator can be used to fertilize eggs from superovulated females. Embryos are incubated overnight to the 2-cell stage, and then surgically re-implanted into pseudopregnant females.
- superovulate C57BL/6 donor females to produce eggs
- generate pseudopregnant recipient females
- thaw frozen sperm sample or collect fresh sperm from donor male
- surgically implant fertilized embryos into pseudopregnant females
- monitor pregnant females
- wean pups
- The recipients will be tested for pathogens before the pups are transferred to your colony.
Prices and Guarantees
- The cost of IVF for mouse rederivation includes thawing frozen sperm or harvesting sperm from a donor male.
- The investigator is responsible for cage per diem costs.
- If the recipients do not become pregnant for any reason, we will repeat the procedure at no additional cost.
- Some inbred strains do not perform well for IVF.
- Click here for current rates.
Reserve and IVF Session